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| 产品名称： | pT7.7/hIL-6 |
|---|---|
| 商品货号： | TS147383 |
| Designations： | pT7.7/hIL-6 |
| Species： | Homo sapiens, human |
| Depositors： | G Ciliberto |
| Applications： | It is recommended that TS147383 be used instead of ATCC 68507. Because the host expresses T7 RNA polymerase, and the cDNA is inserted downstream of the phi10 T7 promoter, this strain shows high levels of expression. |
| Vector： | Construct size (kb): 3.924999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.450000047683716 Gene product: interleukin 6 (interferon, beta 2)(interleukin 6) IL6 |
| Insert Size (kb)： | 1.450 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI/BamHI--2.5, 0.75, 0.5; HincII--2.8, 0.85; BglII--3.7, HindIII--3.7, BglII/BamHI--2.2, 1.5. The XbaI site is upstream of the cDNA insert so excision with this enzyme will include some vector sequence. A new deposit was made for this clone. It is recommended that TS147383 be used instead of ATCC 68507. Contains 1000 bp of the coding region of mature hIL-6 plus 445 bp of the 3 untranslated sequence. Because the host expresses T7 RNA polymerase, and the cDNA is inserted downstream of the phi10 T7 promoter, this strain shows high levels of expression. The product is the mature protein and does not require in vitro cleavage of a fusion protein. |
| References： | Gennaro Ciliberto, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |