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| 产品名称： | pGIKS-CRE |
|---|---|
| 商品货号： | TS147385 |
| Designations： | pGIKS-CRE |
| GenBank Number： | X03453 |
| Species： | Staphylococcus aureus subsp.aureus bacteriophage P1 |
| Depositors： | M Follettie |
| Applications： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector： | Construct size (kb): 3.9 Vector end (5): KpnI Vector end (3): SacI Excise insert: XhoI+SmaI Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 |
| Insert： | DNA: genomic Insert lengths(kb): 1.0 Gene product: recombinase, site-specific cre Insert end (5): KpnI linker Insert end (3): SacI |
| Insert Size (kb)： | 1.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 485-1516 of Genbank X03453. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.36; PvuI--0.46; ClaI--0.81. The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI. Restriction digests of the clone give the following sizes (kb): SmaI--4.0; SmaI/XhoI--3.0, 1.1; XhoI--4.0. |