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| 产品名称： | pCMM8.1 |
|---|---|
| 商品货号： | TS147386 |
| Designations： | pCMM8.1 |
| Species： | Homo sapiens, human |
| Applications： | Prehybridization and hybridization should be carried out in the presence of 250 ug/ml total human DNA. RFLPs are observed under otherwise normal conditions of hybridization and washing. |
| Vector： | Construct size (kb): 17.20000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 14.5 Gene product: DNA Segment, single copy D1S63 Alleles: A1, A2 |
| Insert Size (kb)： | 14.5 |
| Related Products： | component of:ATCC 77175 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--6.6, 4.6, 3.6, 1.5, + smaller; HindIII--9.4, 6.6, 1.7; EcoRI--6.0, 4.0, 2.7, 2.4, 1.5, + smaller; BamHI--16.5, 1.1; SalI--17.0. Codominant segregation demonstrated in 49 3-generation families. Detection of the MspI polymorphism (alleles 1.6 and 0.6 kb) was verified by CEPH. Prehybridization and hybridization should be carried out in the presence of 250 ug/ml total human DNA. RFLPs are observed under otherwise normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII, RsaI, TaqI. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMM8.1) on chromosome 1p D1S63. Nucleic Acids Res. 16: 9370, 1988. PubMed: 2902597 Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294 |