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| 产品名称： | YCpIF10 |
|---|---|
| 商品货号： | TS147388 |
| Designations： | YCpIF10 |
| Depositors： | RW Davis, PK Foreman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1999998092651370 Vector: YCpIF10 (plasmid) Promoters: Promoter for expression GAL1 Construction: pUN15 Marker(s):TRP1,ampR Construct size (kb): 5.199999809265137 Features: marker(s): TRP1 marker(s): ampR promoter for expression: GAL1 replicon: ARS1 replicon: pMB1 MCS: SacI...SalI centromere: CEN4 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 1.7; BamHI--5.2; EcoRI--5.2. Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 1.7; BamHI--5.2; EcoRI--5.2. One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter. Vectors YCpIF10, YCpIF11 and YCpIF12 (TS147388-87089) differ only in the reading frame of the multiple cloning site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907 |
| Related Products： | component of:ATCC 87133 |
| Shipped： | freeze-dried |