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| 产品名称： | pTC4.5 |
|---|---|
| 商品货号： | TS147420 |
| Designations： | pTC4.5 |
| GenBank Number： | L22484 |
| Species： | Trypanosoma cruzi Chagas |
| Depositors： | LM Ruiz-Perez |
| Vector： | Construct size (kb): 7.190000057220459 |
| Insert： | DNA: genomic Insert lengths(kb): 4.5 Gene product: dihydrofolate reductase-thymidylate synthase DHFR-TS |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 2.8; HindIII--7.4; SalI--7.4. The insert contains the following restriction sites (approximate from the 5 end): BstXI--3.95; NcoI--2.52; SalI--2.48; SphI--2.42, 2.76, 3.65; XhoI--2.79. The insert contains the following components (listed 5->3): a PstI/SalI fragment from the MCS of lambda EMBL3, 2.4 kb upstream of the coding sequence for DHFR-TS, the 1.56 kb coding region, and 0.44 kb downstream of DHFR-TS. All but 100 bp of the coding sequence is in a 1.9 kb SalI/EcoRI fragment. |
| Classification： | KINGDOM: Protozoa |
| References： | Reche P, et al. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol. Biochem. Parasitol. 65: 247-258, 1994. PubMed: 7969266 |