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| 产品名称： | HSP-pUC18 |
|---|---|
| 商品货号： | TS147454 |
| Designations： | HSP-pUC18 |
| GenBank Number： | X03168 |
| Species： | Homo sapiens, human |
| Depositors： | DW Barnes, M Solem |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.539999961853027 Tissue: hepatocellular carcinoma Hep G2 cell line Gene product: vitronectin (serum spreading factor, somatomedin B, complement S-protein) VTN |
| Insert Size (kb)： | 1.540 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--1.5, 2.7; HindIII--4.2; BamHI--4.2; PstI--4.2; EcoRI/HindIII--1.5, 2.7. Restriction sites in the insert are consistent with the GenBank/EMBL sequence. The insert, which is not cut by HindIII, SmaI, PstI or BamHI, contains the following restriction sites (nt from the 5 end): KpnI--637, 1047; PvuII--996. Detects the following human genomic restriction fragments under stringent conditions (kb): EcoRI--20-21; HindIII--17-18; BamHI--9-9.5; PstI--2.3-2.5; BglII--13-14. Detects the following murine genomic restriction fragments (kb): EcoRI--7-7.5; HindIII--5.5-6.0; BamHI--3.5-4.0. Includes nucleotides 68-1573 of the GenBank sequence. The coding sequence is from nucleotide 62-1498 of the GenBank record; the signal peptide sequence is from nucleotide 62-118 and the mature peptide is from nt 119-1495. |
| References： | Solem M, et al. Human and mouse S-protein mRNA detected in Northern blot experiments and evidence for the gene encoding S-protein in mammals by Southern blot analysis. Mol. Cell. Biochem. 100: 141-149, 1991. PubMed: 2008176 |