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| 产品名称： | pdhfr3.2 |
|---|---|
| 商品货号： | TS147523 |
| Designations： | pdhfr3.2 |
| Depositors： | GF Crouse |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.4000000953674320 Vector: pdhfr3.2 (plasmid) Construction: pBR322, mouse Marker(s):ampR,mtxR Construct size (kb): 6.400000095367432 Features: marker(s): ampR, mtxR replicon: pMB1 |
| Applications： | expression vector shuttle vector |
| Comments： | Vector which expresses mouse dihydrofolate reductase in eukaryotic cell lines. The Dhfr minigene is selectable in dhfr- cell lines. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Crouse GF, et al. Expression and amplification of engineered mouse dihydrofolate reductase minigenes. Mol. Cell. Biol. 3: 257-266, 1983. PubMed: 6835212 |
| Shipped： | freeze-dried |