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微信小章| 产品名称： | G-Olig2 |
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| 商品货号： | TS147535 |
| Organism： | Mus musculus, mouse |
| Tissue： | inner cell mass |
| Cell Type： | embryonic stem cell |
| Product Format： | frozen |
| Morphology： | spherical colony |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | 129X1/SvJ |
| Applications： | G-Olig2 cells were designed by the insertion of green fluorescent protein (GFP) into the gene for Olig2, a lineage-specific transcription factor.This insertion permits visualization and physical separation of a subset of living ES-cell-derived neural cells.
G-Olig2 cells were designed by the insertion of green fluorescent protein (GFP) into the gene for Olig2, a lineage-specific transcription factor. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SCRC-1037 is a subclone of a deposited cell line. |
| Comments： | G-Olig2 cells were designed by the insertion of green fluorescent protein (GFP) into the gene for Olig2, a lineage-specific transcription factor. This insertion permits visualization and physical separation of a subset of living ES-cell-derived neural cells. PubMed: 12529550 SCRC-1037 is a subclone of deposited cell line. |
| Complete Growth Medium： | Grow ES cells in Mouse ES Cell Basal Medium (ATCC SCRR-2011) that has been supplemented with the following components: 1. 0.1 mM 2-mercaptoethanol (Life Technologies Cat. No. 21985-023) 2. 1,000 U/mL mouse leukemia inhibitory factor (LIF) (Millipore Cat. No. ESG1107) 3. 10% to 15% ES-Cell Qualified FBS (ATCC® SCRR-30-2020) or an ES cell qualified serum replacement Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C. |
| Subculturing： | Subculturing Procedure Note: To insure the highest level of viability, pre-warm media and Trypsin/EDTA to 37ºC before adding to cells. Volumes used in this protocol are for T75 flasks. Proportionally adjust the volumes for culture vessels of other sizes. A split ratio of 1:4 to 1:7 is recommended. Feeder Cell Preparation for Subcultures
Dissociation and Transfer of ES Cells
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| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO.
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | DI Gottlieb |
| Year of Origin： | August, 2001 |
| References： | Matise M, et alProduction of targeted embryonic stem cell clonesIn: Matise M, et alGene Targeting: A Practical ApproachOxfordOxford University Press101-132, 1999 Xian HQ, et al. A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells 21: 41-49, 2003. PubMed: 12529550 |