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微信小章| 产品名称： | S42 |
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| 商品货号： | TS147548 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | sciatic nerve |
| Cell Type： | Schwann cell |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | neonatal |
| Applications： | In many respects, S42 (TS147548) and S16 (ATCC CRL-2941) resemble Schwann cells at an early stage in their preparation to myelinate and are useful for investigating the cell biology of MAG and other myelin-related components. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The S42 cell line is derived from a primary culture of Schwann cells that was immortalized by repetitive passaging. |
| Antigen Expression： | Myelin-associated glycoprotein (MAG) Galactocerebroside (GalC) |
| Genes Expressed： | laminin,Myelin-associated glycoprotein (MAG),Galactocerebroside (GalC) |
| Cellular Products： | laminin |
| Comments： | S42 is similar to S16 (ATCC CRL-2941), but it has a higher level of High Myelin-associated glycoprotein (MAG) and a longer doubling time. At high density, the S42 cells exhibit numerous processes. Both S42 and S16 cells express galactocerebroside (GalC), sulfatide and P0 glycoprotein, but little or no myelin basic protein.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75xa0cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: The culture flasks should be treated with 0.1mL/cm2 of flask surface area with 15µg/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 twice weekly is recommended.
Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | approximately 48 hours |
| Name of Depositor： | RH Quarles |
| Year of Origin： | 1989 |
| References： | Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958 Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597 Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295 |