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微信小章| 产品名称： | pLOI295 |
|---|---|
| 商品货号： | TS147602 |
| Designations： | pLOI295 |
| Species： | Zymomonas mobilis (Lindner) Kluyver and van Niel |
| Depositors： | University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville |
| Applications： | produces protein alcohol dehydrogenase II produces protein pyruvate decarboxylase |
| Vector： | Construct size (kb): 5.867000102996826 |
| Insert： | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: alcohol dehydrogenase II pdc Target Gene: pyruvate decarboxylase, alcohol dehydrogenase II |
| Insert Size (kb)： | 3.200 |
| Media： | 1227 plus 2% glucose
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze driedxa0 E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.7, 3.2; BglI--2.5, 1.3, 1.1, 0.9; HindIII--3.1, 1.7, 0.8, 0.2. The GenBank accession number for the adh insert is M15394. Expression appears to be regulated by the lac promoter, and growth is increased under anaerobic conditions. Constructed by inserting a 1.4 kb EcoRI/SalI fragment from pLOI284 containing the promoterless adh coding sequence into the BamHI site of pLOI276. The pdc reading frame begins 163 bp downstream of the lac promoter and ends with two stop codons 85 bp upstream of the adhB reading frame. The adhB gene has a single stop codon followed by a palindromic sequence which serves as a terminator. pLOI284 consists of two DraI fragments of 0.12 and 1.23 kb cloned into the SmaI site of pUC18. The insert starts at the DraI site 402 nt downstream of the 5 DraI site of the reported sequence, with the coding region from nt 30 to 1181 of the insert. The (5)EcoRI/BamHI (3) fragment contains both genes. |
| References： | Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991 Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063 Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037 Ingram LO, Conway T. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 54: 397-404, 1988. Ingram LO, et al. Genetic engineering of ethanol production in Escherichia coli. Appl. Environ. Microbiol. 53: 2420-2425, 1987. PubMed: 3322191 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |