| 产品名称: | pmigf2-3 |
|---|---|
| 商品货号: | TS147630 |
| Designations: | pmigf2-3 |
| GenBank Number: | M14951 |
| Species: | Mus musculus, mouse |
| Depositors: | GI Bell |
| Vector: | Construct size (kb): 4.75 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.447999954223633 Tissue: placenta (19 day) Gene product: insulin-like growth factor 2 Igf2 |
| Insert Size (kb): | 1.448 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | The insert includes these restriction sites (nucleotide position according to the published sequence): BamHI--45, 1075; SacI--473. Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 1.5; HindIII--4.6; BamHI--2.7, 1.0, 0.7; PstI--4.6; SacI--4.6. This contains the complete coding sequence, plus a 5 untranslated region of 31 bp and a 3 untranslated region of 864 bp. This detects a major placental transcript of 3.85 kb and a minor transcript of 4.8 kb. It did not hybridize to adult liver poly(A)+ RNA. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Stempien MM, et al. Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor. DNA 5: 357-361, 1986. PubMed: 3780370 |