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| 产品名称： | pFBJ-MLV-1 |
|---|---|
| 商品货号： | TS147655 |
| Designations： | pFBJ-MLV-1 |
| Species： | Murine osteosarcoma virus |
| Depositors： | T Curran |
| Vector： | Construct size (kb): 12.60000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 8.199999809265137 Tissue: DNA from mouse BALB/c NIH 3T3 cell line |
| Insert Size (kb)： | 8.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb): HindIII- 1.1- PstI- 2.0- PstI(in the LTR)- 3.6- XhoI- 0.6- SalI- 0.3- SstI- 0.6- HindIII. Restriction digests of the clone give the following sizes (kb): HindIII--8.2, 4.4; XhoI--12.6. This contains an entire copy of FBJ-MLV, circularly permuted with a single LTR. Digestion by HindIII and ligation will reconstitute an infectious viral DNA. Unintegrated supercoiled DNA. MARKER: Ap(r). This clone is circularly permuted with a single LTR. HindIII digestion followed by ligation reconstitutes an infectous viral DNA. NIH 3T3 cells infected with biologically cloned FBS-MLV (see Curran and Teich, Virology 116: 221-235, 1982) Unintegrated supercoiled DNA. MARKER: Ap(r). This clone is circularly permuted with a single LTR. Hind III digestion followed by ligation reconstitutes an infectous viral DNA. |
| References： | Curran T, et al. Structure of the FBJ murine osteosarcoma virus genome: Molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells. Mol. Cell. Biol. 3: 914-921, 1983. PubMed: 6306448 |