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| 产品名称： | lambdaEnk1 |
|---|---|
| 商品货号： | TS147686 |
| Designations： | lambdaEnk1 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 47.0 |
| Insert： | DNA: genomic Insert lengths(kb): 16.0 Tissue: fetal liver Gene product: proenkephalin PENK Alleles: A1, A2 |
| Insert Size (kb)： | 16.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | EcoRI digest contains fragments of about 20, 11, 6.8, 3.2, 2.6, 1.23, 1.15 and 1.07 kb. Restriction digests of the clone give the following sizes (kb): BamHI--29, 6.4, 5.6, 4.0, 1.6, 0.54; EcoRI--20, 11, 6.8, 3.2, 2.7, 1.3, 1.25, 1.1; HindIII--20, 5.8, 5.8, 4.25, 3.25, 2.7, 2.3, 1.9, 1.15; SalI--uncut; XbaI--20, 15, 12. This genomic clone contains the entire coding region of proenkephalin. To block repeated sequences in the probe, prehybridize with sonicated total human placental DNA instead of salmon sperm DNA. Final stringency wash with 0.1X SSC/0.1% SDS at 65-68C. Enzyme(s) not detecting polymorphism: 29 enzymes including TaqI, MspI, RsaI, BamHI, PvuII, PstI. |
| References： | Comb M, et al. Primary structure of the human proenkephalin gene. DNA 2: 213-229, 1983. PubMed: 6315326 Litt M, et al. Chromosomal localization of the human proenkephalin and prodynorphin genes. Am. J. Hum. Genet. 42: 327-334, 1988. PubMed: 2893547 Litt M, Buder A. A frequent RFLP identified by a human proenkephalin genomic clone (HGM9 symbol PENK). Nucleic Acids Res. 17: 465, 1989. PubMed: 2783483 James Douglass, personal communication |