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| 产品名称： | pCN38 |
|---|---|
| 商品货号： | TS147706 |
| Designations： | pCN38 |
| Depositors： | R Diaz Orejas |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0999999046325680 Vector: pCN38 (plasmid) Construction: pPS10, pKT231 Marker(s):kanR Construct size (kb): 4.099999904632568 Features: marker(s): kanR replicon: repA |
| Applications： | Compatible with broad-host-range plasmids of the incompatibility groups P1, P4/Q and W, and maintained at a copy number of 15-20 in P. savastanoi. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.95; HindIII--4.2; BamHI/KpnI--2.2, 2.0. Compatible with broad-host-range plasmids of the incompatibility groups P1, P4/Q and W, and maintained at a copy number of 15-20 in P. savastanoi. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences. General purpose vector. Derived from the ligation product of a 7.6 kb PstI fragment of pPS10 and a 4.2 kb PstI fragment of pKT231 which was then shortened by deletion. A 1.8 kb region of pPS10 was retained (EMBL accession number X58896) which encodes replication functions. The order of the major features in this plasmid is: PvuII - BamHI - kanR - HindIII - KpnI - repA. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095 Ramon Diaz Orejas, personal communication |
| Shipped： | freeze-dried |