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| 产品名称： | HuBS3-Abeta (NH2) M29551, SGC31484 |
|---|---|
| 商品货号： | TS147718 |
| Designations： | HuBS3-Abeta (NH2) M29551, SGC31484 |
| GenBank Number： | M29551 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 44.90000152587891 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lambdagt11 Intact vector size: 43.700 Type of vector: phage Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Cloning capacity: 7.2 kb Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5) Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ promoter: lac replicon: lambda repressor gene: cI857 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PPP3CB Other gene symbols: CALNA2 Genomic copy number: unique Gene name: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta) Contains complete coding sequence?: U Chromosome: 10; Localization: 10; 10, 448.339999 cR Tissue: brainstem Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.215 Cross references: DNA Seq. Acc.: M29551 Insert lengths(kb): 1.2150000333786 Tissue: brainstem Gene product: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)(protein phosphatase 3, catalytic subunit, beta isoform) CALNA2 |
| Insert Size (kb)： | 1.215 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--24.2, 19.5, 1.2; ClaI--12.5, 6 (doublet), 4.2, 4.0, 2.7, 2.6, 2.0, 1.85, 1.1 (doublet), 0.95, 0.6, 0.35; HindIII-->23.0, 9.4, 6.2, 3.8, 0.56; SmaI--19.5, 10.5, 8.3, 5.8; SstI--20.0, 18.5, 4.45, 1.35. The insert contains the following restriction sites (approximate kb from the 5 end): ClaI--0.97; HindIII--0.67, 0.68; NcoI--0.42; SacI--0.98; SmaI--0.10, 0.14, 0.18. Corresponds to nt 1-1215 of the GenBank record, including the 5 portion of the coding region (nt 117-1691). This is the sequence for the more prevalent type II isoform, and lacks 54 bp in this region relative to the type I. |
| References： | Guerini D, Klee CB. Cloning of human calcineurin A: evidence for two isozymes and identification of a polyproline structural domain. Proc. Natl. Acad. Sci. USA 86: 9183-9187, 1989. PubMed: 2556704 |