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| 产品名称： | pAR3 |
|---|---|
| 商品货号： | TS147738 |
| Designations： | pAR3 |
| Depositors： | J Gutierrez |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH1 |
| Vector Information： | Size (kb): 3.32 DESCRIPTION OF VECTOR: Intact vector size: 3.320 Type of vector: plasmid Cloning sites: PstI NarI BglII KpnI HindIII Polylinker sites: NcoI PstI NarI EcoRV BglII KpnI HindIII Construction: pACYC184, pARA12 Host range: Escherichia coli Features (with orientation and position when available): replicon: p15A MCS: HindIII...NcoI, -> promoter: paraB, coding sequence: araC, -> marker(s): cmLR, Vector: pAR3 (plasmid) Promoters: Promoter paraB Construction: pACYC184, pARA12 Marker(s):cmlR Construct size (kb): 3.32 Features: marker(s): cmlR promoter: paraB replicon: p15A MCS: HindIII...NcoI coding sequence: araC |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--3.4; EcoRV--2.5, 0.45 (doublet); PstI--3.4. This vector is compatible with ColE1-derived plasmids and allows efficient expression of recombinant genes upon induction with arabinose. These features make it convenient to be used in combination with standard gene expression vectors for the independently controlled production of two recombinant polypeptides in E. coli. |
| Media： | ATCC® Medium 2272: LB Medium (Miller) with chloramphenicol 25 mcg/ml |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cagnon C, et al. A new family of sugar-inducible expression vectors for Escherichia coli. Protein Eng. 4: 843-847, 1991. PubMed: 1798708 Perez-Perez J, Gutierrez J. An arabinose-inducible expression vector, pAR3, compatible with colE1-derived plasmids. Gene 158: 141-142, 1995. PubMed: 7789799 Julio Gutierrez, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |