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微信小章| 产品名称： | Neospora caninum Dubey et al. |
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| 商品货号： | TS147867 |
| Strain Designations： | NC-Beef |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clinical specimen - animal Nebraska, United States Isolation date: 1998 |
| Product Format： | frozen |
| Storage Conditions： | liquid nitrogen vapor phase |
| Type Strain： | no |
| Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 95%; horse serum, 5%
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| Growth Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Duration: The organism is propagated in Vero cells. Protocol:
Interval: approximately 1 week |
| Subcultivation： | Protocol:
Interval: approximately 1 week |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | MM McAllister |
| Special Collection： | NSF - Protistology |
| Year of Origin： | 1998 |
| References： | Barber JS, et al. Characterization of the first European isolate of Neospora caninum (Dubey, Carpenter, Speer, Topper and Uggla). Parasitology 111: 563-568, 1995. PubMed: 8559588 Gondim LF, et al. Variation of the internal transcribed spacer 1 sequence within individual strains and among different strains of Neospora caninum. J. Parasitol. 90: 119-122, 2004. PubMed: 15040677 McAllister MM, et al. Evidence of point-source exposure to Neospora caninum and protective immunity in a herd of beef cows. J. Am. Vet. Med. Assoc. 217: 881-887, 2000. PubMed: 10997162 beef calf (Bos taurus) |
| Cross References： | Nucleotide (GenBank) : AY259040 Neospora caninum strain NC-beef internal transcribed spacer 1, complete sequence |