微信小陈
微信小章
产品简介
购买须知
| 产品名称： | phrosHi6 |
|---|---|
| 商品货号： | TS147872 |
| Designations： | phrosHi6 |
| Species： | Homo sapiens, human |
| Depositors： | K Huebner, L Nagarajan |
| Vector： | Construct size (kb): 8.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 5.5 Tissue: promyelocytic leukemia Gene product: avian UR2 sarcoma virus oncogene (v-ros) homolog ROS Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 5.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 2.3; HindIII--5.5, 2.7; PstI--4.8, 2.1, 1.1; BamHI--5.6, 2.2; AccI--3.7, 2.9, 1.5. The insert contains SacI (2) and BamHI (1) sites. The depositor indicates a similar clone has been sequenced in another lab. This probe spans a large part of the region homologus to v-ros. Allele A1 was seen in 1/39 cell lines; A2 in 37/39 cell lines, and both present in 1/39. PstI digestion of genomic DNA gives 3 bands of 5.3, 3.2, 1.3 kb in 19/19 cell lines. An additional band of 2.65 kb was detected in 1 of the 19. Enzyme(s) not detecting polymorphism: BamHI, EcoRV, SstI. The genomic fragment sizes detected are (kb): BamHI--11, 3.15; EcoRV--12; SstI--greater than 30, 16, 1.5. |
| References： | Matsushime H, et al. Human c-ros-1 gene homologous to the v-ros sequence of UR2 sarcoma virus encodes for a transmembrane receptorlike molecule. Mol. Cell. Biol. 6: 3000-3004, 1986. PubMed: 3023956 Nagarajan L, et al. The human c-ros (ROS) gene is located at chromosome region 6q16----6q22. Proc. Natl. Acad. Sci. USA 83: 6568-6572, 1986. PubMed: 3529088 |