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微信小章| 产品名称： | HuT 78 |
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| 商品货号： | TS147889 |
| Organism： | Homo sapiens, human |
| Cell Type： | cutaneous T lymphocyte (KRAS CRM) |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Sezary Syndrome |
| Age： | 53 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.xa0
This cell line is suitable as a transfection host. |
| Intended Use： | Certified Reference Material produced under an ISO 17034 accredited process.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. |
| Clinical Data： | 53 years adult Caucasian male |
| Antigen Expression： | CD4; Homo sapiens Ref   Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 |
| Receptor Expression： | interleukin 2 (IL-2), expressed Ref Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 |
| Genes Expressed： | interleukin 2, tumor necrosis factor alpha (TNF alpha), CD4; Homo sapiens |
| Cellular Products： | interleukin 2 tumor necrosis factor alpha (TNF alpha) |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice when injected intracranially |
| Comments： | Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Certified Reference Material produced under an ISO 17034 accredited process. Item has been tested for KRAS mutation; KRAS wildtype The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 105 viable cells/mL. Maintain cultures at cell concentrations between 5 X 104 and 8 X 105 viable cells/mL. maintain cell density at less than 1 X 106 cells/mL.
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze Medium: Complete growth medium 95%; DMSO, 5% Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11,12 D5S818: 11,12 D7S820: 8,11 THO1: 8,9 TPOX: 8,9 vWA: 14,15 |
| Population Doubling Time： | about 65 hours |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| References： | Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177 Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674 OConnell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285 Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 |
