宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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CRI-R99

货号 TS147915
中文名称 null
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产品名称: CRI-R99
商品货号: TS147915
Designations: CRI-R99
Species: Homo sapiens, human
Depositors: Collaborative Research, Inc.
Vector:
Construct size (kb): 47.79999923706055
Insert:
DNA: genomic
Insert lengths(kb): 17.29999923706055
Tissue: liver
Gene product: DNA Segment, single copy D16S75
Alleles: A1, A2, A3, B1, B2
Insert Size (kb): 17.300
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--23.1, 13.1, 8.4, 5.3, 2.4, 1.24; BglI--23.1, 9.9, 5.1, 5.0, 2.2, 2.1, 1.8, 1.39, 0.71; BamHI--19.1, 13.7, 7.9, 6.0, 4.1, 2.6, 1.6, 1.11;
PstI--14.4, 9.8, 5.6, 4.1, 3.3, 3.1, 2.8 (doublet), 2.1, 1.83, 1.35, 1.25, 1.16; HindIII--27.9, 6.0, 3.7, 1.67, 1.33, 0.94, 0.93.
The HindIII and MspI polymorphisms have a combined PIC value of 0.62 and a combined heterozygosity of 64%.
Enzyme(s) not detecting polymorphism: BglII, EcoRI, HincII, BamHI, PstI, RsaI, TaqI (panel of 5 individuals).
References:

Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638

Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474