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| 产品名称： | phgH6 hGH6 |
|---|---|
| 商品货号： | TS147921 |
| Designations： | phgH6 hGH6 |
| GenBank Number： | X13461 |
| Species： | Homo sapiens, human |
| Depositors： | EE Strehler |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 2.733999967575073 Tissue: leukocyte Gene product: calmodulin-like CALML3 |
| Insert Size (kb)： | 2.734 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.15; PvuII--2.4, 1.1, 0.95, 0.75, 0.35; SacI--2.7 (doublet); SacI/BglI--1.4, 1.15, 0.9, 0.85, 0.65, 0.4; SmaI--3.7, 1.2, 0.75; SmaI/SacI--2.7, 1.2, 0.9, 0.75. The insert contains the following restriction sites (nt from the 5 end): PstI--106, 752, 1741, 1905, 1998, 2208; PvuII--162, 1077, 2144; SmaI--863, 1569; NotI--953; EcoRI--1099. Contains a complete open reading frame of 444 nucleotides, with 5 and 3 flanking sequences. A 0.7 kb SmaI fragment containing the coding region (nt 863 -1569) detects a 2.3 kb mRNA in human erythroleukemia K562 cells. A 1.2 kb SmaI/SacI fragment from the 3 region (nt 1570 - 2738) detects a 1.5 kb mRNA in SV40-transformed human fibroblasts. The intact insert can be separated from the vector by a SacI/SmaI double digest. |
| References： | Koller M, Strehler EE. Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett. 239: 121-128, 1988. PubMed: 3181418 |