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| 产品名称： | p680 |
|---|---|
| 商品货号： | TS147971 |
| Designations： | p680 |
| Depositors： | F Spencer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.9000000953674320 Vector: p680 (plasmid) Promoters: Promoter for in vitro transcription T7 Marker(s):ampR Construct size (kb): 6.900000095367432 Features: insert detection: lacZ marker(s): ampR promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: f1(+) replicon: ori coding sequence: CYH2 coding sequence: LEU2 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 2.2, 1.4; EcoRI--3.1, 2.0, 1.8; KpnI--5.2, 1.8; PvuI--5.0, 2.0. Vector contains the following restriction sites: EcoRV--1.01,3.20,5.64; BstXI--1.04,3.15; EcoRI--1.12,5.94; KpnI--1.51,3.23; ClaI--1.60,3.30; NacI--2.79; PvuI--2.97,4.88; PvuII--3.00,3.45; ScaI--3.12,4.99; AatII--5.44. Two-step replacement in budding yeast. LEU2 gene provides positive selection for the integrative step; CYH2S provides negative selection for mitotic recombinants that have excised the plasmid sequences. Can be used for modification of YACs. The fragments were ligated in the plasmid: LEU2 -> f1(+) -> lacZ -> T7/T3 -> ori -> ampR -> CYH2 ... |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993. |
| Shipped： | freeze-dried |