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| 产品名称： | pXPGK-RI0.9 |
|---|---|
| 商品货号： | TS148066 |
| Designations： | pXPGK-RI0.9 |
| Species： | Homo sapiens, human |
| Applications： | The smaller EcoRI fragment has been used as the probe. |
| Vector： | Construct size (kb): 5.260000228881836 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: phosphoglycerate kinase 1 PGK1 Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 0.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 0.74; PvuII--4.9; EcoRI/PvuII--2.4, 1.5, 0.79; BamHI--uncut; HindIII--uncut; AccI--3.3, 1.7. This PstI RFLP is in strong linkage disequilibrium (87% of maximal) with the 5 BglI RFLP detected at this locus by pSPT-PGK. Insert contains the last 83 bp of exon 4 and extends 820 bp into the fourth intron of human PGK1. Occasionally a faint 5.2 kb autosomal band is detected with the PstI RFLP. The smaller EcoRI fragment has been used as the probe. Based on the restriction data and the tetracycline sensitivity of this construct, the vector must not be the reported pBR322. |
| References： | Smead DL, et al. RFLPs in human X-linked PGK1: A new probe for the PstI RFLP demonstrates strong linkage disequilibrium with the BglI RFLP. Nucleic Acids Res. 17: 7551, 1989. PubMed: 2571982 Merry DE, et al. Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21. Am. J. Hum. Genet. 45: 530-540, 1989. PubMed: 2491012 Jennifer M Puck, personal communication |