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| 产品名称： | pEFZ18.2 |
|---|---|
| 商品货号： | TS148125 |
| Designations： | pEFZ18.2 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | EcoRI/HindIII--2.8, 2.5, 1.2, 0.5. Shows same pattern as DNA used for transformation. |
| Vector： | Construct size (kb): 7.0 |
| Insert： | DNA: genomic Insert lengths(kb): 4.199999809265137 Gene product: DNA Segment, single copy D14S17 Alleles: T1, T2 |
| Insert Size (kb)： | 4.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 2.4; HindIII--5.3, 1.2; EcoRI/HindIII--2.8, 2.5, 1.2, 0.5. Shows same pattern as DNA used for transformation. An EcoRI/HindIII double digest shows insert fragment sizes of 2.3, 1.0, and 0.4 kb. Codominant segregation of the TaqI polymorphism has been demonstrated in 54 3-generation families. Enzyme(s) not detecting polymorphism: MspI, RsaI. |
| References： | Fujimoto E, et al. Isolation and mapping of a polymorphic DNA sequence pEFZ18.2 on chromosome 14 D14S17. Nucleic Acids Res. 16: 380, 1988. PubMed: 2893344 Yusuke Nakamura, personal communication |