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| 产品名称： | pB34BSM |
|---|---|
| 商品货号： | TS148144 |
| Designations： | pB34BSM |
| Species： | Beet necrotic yellow vein virus |
| Depositors： | KE Richards |
| Vector： | Construct size (kb): 3.940000057220459 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7400000095367432 Gene product: genome, partial |
| Insert Size (kb)： | 0.740 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--2.9, 1.0; EcoRI--3.9; BamHI--4.2. To synthesize antisense RNA in vitro, cut with BamHI and transcribe from vector T3 promoter. The insert is nt 1-740 of RNA-3 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious. |
| Classification： | Furovirus |
| References： | Bouzoubaa S, et al. Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. J. Gen. Virol. 66: 1553-1564, 1985. Ken E Richards, personal communication |