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| 产品名称： | Singhamoeba horticola (Singh and Hanumaiah) Sawyer et al. |
|---|---|
| 商品货号： | TS148188 |
| Deposited As： | Schizopyrenus horticolus Singh and Hanumaiah |
| Strain Designations： | CR-1-3 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | bottom sediment, Chester River, MD, 1985 |
| Product Format： | dried |
| Type Strain： | no |
| Comments： | species description |
| Medium： | ATCC® Medium 802: Sonneborns Paramecium medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Name of Depositor： | TK Sawyer |
| Year of Origin： | 1985 |
| References： | Sawyer TK, et al. Singhamoeba horticala new combination Singh and Hanumaiah 1979 type species of Singhamoeba new genus. J. Protozool. 39: 107-109, 1992. |