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| 产品名称： | pCYM3 |
|---|---|
| 商品货号： | TS148227 |
| Designations： | pCYM3 |
| Depositors： | JH Camonis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5430002212524410 Vector: pCYM3 (plasmid) Promoters: Promoter SV40 early Construction: pCYM2 (ATCC 37824), pTG2586 Marker(s):TRP1,ampR,URA3d Construct size (kb): 6.543000221252441 Features: marker(s): ampR, TRP1, URA3d promoter: SV40 early replicon: pMB1, 2 micron terminator: SV40 |
| Applications： | YE-type (episomal) shuttle vector expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--uncut; ClaI/BamHI--3.4, 2.8, 0.5; EcoRI--6.6; BamHI/PvuII--6.0, 0.5. URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation. Shuttle expression vector. Permits expression in both S. cerevisiae and mouse cells. The EcoRI, SmaI, and BamHI sites are 3 to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by inserting a 1.207 kb ClaI/BamHI fragment containing URA3d into pCYM2 cut with BglII and ClaI (nt 3469 and 3589 according to the pCYM0 numbering scheme). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907 |
| Shipped： | freeze-dried |