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| 产品名称： | pTAM2.5-a |
|---|---|
| 商品货号： | TS148236 |
| Designations： | pTAM2.5-a |
| GenBank Number： | J03520 |
| Species： | Mus musculus, mouse |
| Depositors： | S Strickland |
| Vector： | Construct size (kb): 5.480000019073486 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.519000053405762 Tissue: teratocarcinoma F9 cell line + parietal endoderm Gene product: plasminogen activator, tissue Plat |
| Insert Size (kb)： | 2.519 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7, 0.8; BamHI--5.5; XbaI--4.3, 1.25; HindIII--5.5; XhoI--5.5; BamHI+HindIII--3.0, 2.5. Transcription from the vector T7 promoter yields sense transcripts. Full-length cDNA clone which includes 87 bp of 5 non-coding sequence and 745 bp of 3 non-coding sequence. This clone was constructed by Bill Richards from the 5 end of clambdaEPE (parietal endoderm) and the EcoRI site to the 3 end of clambda2 (F9 teratocarcinoma). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Rickles RJ, et al. Molecular cloning of complementary DNA to mouse tissue plasminogen activator mRNA and its expression during F9 teratocarcinoma cell differentiation. J. Biol. Chem. 263: 1563-1569, 1988. PubMed: 2826484 Sidney Strickland, personal communication |