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微信小章| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
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| 商品货号： | TS148240 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Strain Designations： | BDMS 18 AN |
| Application： | Quality control strain
Quality control strain for Clostridioides difficile agar
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Enteric Research
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| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | Toxinotype: 0 |
| Comments： | Presence of tcdA and tcdB genes confirmed by PCR. Ref
 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
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| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions： | Temperature: 37°C Atmosphere:xa0Anaerobic |
| Name of Depositor： | Becton Dickinson Microbiology Systems |
| Special Collection： | NCRR Contract |
| Chain of Custody： | ATCC |
| References： | Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 |