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产品简介
购买须知
| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS148250 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | RPmuhr23HA+ recombinant of Utrecht strain RPuhr23 |
| Applications： | in suitable host, produces protein hemagglutinin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Vaccinia/rabbitpox recombinant |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | Sequences cloned into pHGN3 (ATCC 69051) can be integrated into this virus by transfection of a virus-infected host. Recombinants can be identified by inactivation of the hemagglutinin gene (HA-). This is a potential human pathogen. Parent strain RPuhr23 is a spontaneous white pock mutant of wild-type rabbitpox. The HA inserted in TS148250 can serve as a cloning site for genes affecting vaccinia and rabbitpox. ATCC 69051 is a shuttle vector for use with this system. Avirulent, hemagglutinin positive (HA+) recombinant, derived from the white pock deletion mutant of rabbit poxvirus. Part of a virulence testing system to be used with pHGN3 (ATCC 69051). The insert contains approximately 443 bp 5 flanking sequence, 988 bp coding sequence and 408 bp 3 flanking sequence. Constructed by introducing the hemagglutinin gene from vaccinia virus IHD-J by homologous recombination. |
| Effect on Host： | Cytopathic effects in tissue culture |
| Name of Depositor： | University of Florida, Gainesville, RW Moyer, University of Florida, Gainesville |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Vaccinia/rabbitpox recombinant |
| References： | Moyer RW, Bloom DC. Biological system for constructing and testing viral vaccines. US Patent 5,212,057 dated May 18 1993 |