微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YRp17 |
|---|---|
| 商品货号： | TS148294 |
| Designations： | YRp17 |
| Depositors： | RW Davis |
| Other IDs： | Nucleotide (GenBank) : VB0069 YRp17 yeast replicating plasmid. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: YRp17 (plasmid) Promoters: Promoter none Construction: YIp5, TRP1-ARS1 Marker(s):TRP1,URA3,ampR,tetR Construct size (kb): 7.0 Features: marker(s): ampR, tetR, URA3, TRP1 promoter: none replicon: pMB1, ARS1 terminator: none enhancer: none |
| Applications： | YR-type (replicating) shuttle vector shuttle vector |
| Comments： | YRp17 transforms yeast at high frequencies and is mitotically unstable. A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector. YRp17 differs from YRp12 by the loss of the EcoRI site adjacent to the ampR gene. YRp12 was constructed from YIp5 (ATCC 37061) by inserting a 1.45 kb EcoRI TRP1-ARS1 fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Mann C, Davis RW. Instability of dicentric plasmids in yeast. Proc. Natl. Acad. Sci. USA 80: 228-232, 1983. PubMed: 6337372 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Shipped： | freeze-dried |