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| 产品名称： | pHUK-8 pHUK-1 |
|---|---|
| 商品货号： | TS148323 |
| Designations： | pHUK-8 pHUK-1 |
| GenBank Number： | K02286 |
| Species： | Homo sapiens, human |
| Depositors： | F Blasi |
| Applications： | Enzyme(s) not detecting polymorphism: EcoRI, HindIII, PstI, PvuII, SmaI, SstI, MspI, TaqI. Tested against 8 unrelated individuals. |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.5 Tissue: SV40-transformed fibroblast Gene product: plasminogen activator, urokinase PLAU Alleles: A1, A2 |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.9, 1.5; BamHI--4.3, 1.2; AvaI--5.6; HindIII--5.8; EcoRI--4.6, 0.63, 0.42. Includes unspliced intervening sequences and 3 non-coding sequences. Encodes from about aa 103 through 270 bp 3 untranslated. This is a subclone of pHUK-1. BamHI RFLP has demonstrated codominant segregation in an informative family of 7. Enzyme(s) not detecting polymorphism: EcoRI, HindIII, PstI, PvuII, SmaI, SstI, MspI, TaqI. Tested against 8 unrelated individuals. |
| References： | Verde P, et al. Identification and primary sequence of an unspliced human urokinase poly(A)+ RNA. Proc. Natl. Acad. Sci. USA 81: 4727-4731, 1984. PubMed: 6589620 Sebastio G, et al. BamHI RFLP linked to the human urokinase gene. Nucleic Acids Res. 13: 5404, 1985. PubMed: 2991863 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 |