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| 产品名称： | pBlueBac/PKCdelta |
|---|---|
| 商品货号： | TS148364 |
| Designations： | pBlueBac/PKCdelta |
| GenBank Number： | L07860 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein protein kinase C, delta |
| Vector： | Construct size (kb): 16.10000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBlueBac Intact vector size: 14.000 Type of vector: phagemid Vector end: NheI Vector end: NheI Cloning sites: NheI Polylinker sites: Host range: insect cells; Escherichia coli Features (with orientation and position when available): insert detection: lac marker(s): ampR promoter: polyhedrin replicon: pMB1, f1, SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: PRKCD Gene name: protein kinase C, delta Contains complete coding sequence?: Y Localization: 3 Tissue: liver, HepG2 cell line Type of DNA: cDNA Insert end: Modification: NheI linkers Insert end: Modification: NheI linkers Insert size (kb): 2.1 Cross references: DNA Seq. Acc.: L07860 Insert lengths(kb): 2.099999904632568 Tissue: liver, HepG2 cell line Gene product: protein kinase C, delta PRKCD Target Gene: protein kinase C, delta |
| Insert Size (kb)： | 2.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.1; BglI--5.6, 5.4, 2.2, 1.8, 1.2; HindIII--8.8, 4.0, 1.3, 1.1, 1.0; NcoI--14.0, 1.25, 0.5, 0.35; PstI--14.0, 1.1, 0.55, 0.3. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--1.13; BglII--1.01; HindIII--0.85; NcoI--1.18, 1.64, 1.96; PstI--0.05, 0.33, 1.39; SalI--0.17. Contains the complete coding sequence, and begins at the initiation codon (nt 59 of the sequence record). |
| References： | Aris JP, et al. Molecular and biochemical characterization of a recombinant human PKC-delta family member. Biochim. Biophys. Acta 1174: 171-181, 1993. PubMed: 8357834 |