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| 产品名称： | pSP65:TPMV #6 full-length clone of tomato planta macho viroid |
|---|---|
| 商品货号： | TS148381 |
| Designations： | pSP65:TPMV #6 full-length clone of tomato planta macho viroid |
| Species： | Tomato planta macho viroid |
| Depositors： | RA Owens, Beltsville Agricultural Research Center |
| Applications： | Noninfectious unless TPMV cDNA insert is released by BamHI. To use as a template, plasmid must be linearized with HindIII. |
| Vector： | Name of construct: pSP65:TPMV #6 Excise insert: BamHI Size of construct (kb): 3.36 Markers: ampR Name of vector: pSP65 Intact vector size: 3.010 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: SP6 replicon: pMB1 terminator: none |
| Insert： | Genome: tomato planta macho viroid Gene name: genome, full-length Contains complete coding sequence?: U Type of DNA: cDNA Insert end: BamHI Insert end: BamHI Insert size (kb): 0.36 |
| Insert Size (kb)： | 0.36 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains an internal SalI site. EcoRI + SalI or HindIII + SalI are diagnostic for the insert orientation. Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 0.36; HindIII--3.4; EcoRI--3.4. Noninfectious unless TPMV cDNA insert is released by BamHI. To use as a template, plasmid must be linearized with HindIII. Full-length (360 bp) TPMV cDNA with BamHI ends was cloned into BamHI of pSP65. Constructed by R.W. Hammond. |
| Classification： | Pospiviroid |
| References： | Kiefer MC, et al. Structural similarities between viroids and transposable genetic elements. Proc. Natl. Acad. Sci. USA 80: 6234-6238, 1983. PubMed: 6312450 Robert A Owens, personal communication |