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| 产品名称： | pOM1 |
|---|---|
| 商品货号： | TS148383 |
| Designations： | pOM1 |
| Depositors： | I Oberbaumer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7160000801086430 Vector: pOM1 (plasmid) Promoters: Promoter lac Construction: pUC9, M13tg131 Marker(s):ampR Construct size (kb): 2.716000080108643 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector for constructing cDNA libraries vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; XhoI--2.7. Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. pOM1 (TS148383) and pOM2 (ATCC 77221) differ in the orientation of the polylinker. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975 Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992. |
| Shipped： | freeze-dried |