Construct size (kb): 5.099999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: P63*
Gene name: transmembrane protein p63, ER-Golgi intermediate
compartment
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: T08176; EST: EST06067; Primer: M13-21; THC
id: THC83089
DNA Seq. Acc.: T08177; EST: EST06068; Primer: M13 Reverse;
THC id: THC83089
DNA Seq. Acc.: X69910; EST: EST06067
Nucleotides 6-365 of the insert correspond to
nucleotides 439-798 of X69910 with 97% identity.
DNA Seq. Acc.: X69910; EST: EST06068
Nucleotides 1-321 of the insert correspond to
nucleotides 1916-2236 of X69910 with 97% identity.

Insert lengths(kb): 1.799999952316284

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: transmembrane protein p63, ER-Golgi intermediate compartment(transmembrane protein p63) P63*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII/NotI--3.2, 1.1, 0.74.

Verified to contain EST06067 by single-pass sequencing.