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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS148425 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | HB101 Flac::Tn1739tnpR |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Shipped: xa0Freeze dried Escherichia coli |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F lac::Tn1739tnpR lacY1 galK2 leuB6 proA2 thi recA13 rpsL20 delta(mcrC-mrr) xyl-5 mtl-1 supE44 |
| Type Strain： | no |
| Comments： | A chloramphenicol resistant host, expressing tnpR resolvase and the cI lambda repressor, for use in the Tn1721-derived site-specific recombination systems and excision of plasmids from the vector lambdaRESII (ATCC 77404). Phenotype verified: gal, lac, strR, leu, pro, thi, supE, F, r-m-, cmlR. Contains two copies of the tnpR gene, one under control of the tac promoter for increased inducible expression. The order of the major features in the transposon is: inverted repeats - EcoRI - tnpR - tac promoter - EcoRI - BamHI - cI - PstI - cmlR - HindIII - res resolution site - tnpR - tnpA - EcoRI - inverted repeats. |
| Medium： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C Atmosphere: aerobic |
| Name of Depositor： | J Altenbuchner |
| References： | Altenbuchner J. A new lambda RES vector with a built-in Tn1721-encoded excision system. Gene 123: 63-68, 1993. PubMed: 8380784 |