微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pSH |
|---|---|
| 商品货号： | TS148441 |
| Designations： | pSH |
| Depositors： | AM Ventura |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.9000000953674320 Vector: pSH (plasmid) Promoters: Promoter T3 Construction: pEN1, BLpSV Marker(s):ampR Construct size (kb): 5.900000095367432 Features: marker(s): ampR promoter: HTLV-1 LTR, T7, T3 replicon: pMB1, SV40 terminator: SV40 polyadenylation enhancer: HIV-1 LTR |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; HindIII--5.9; PstI--4.3, 1.6. Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse cells. The order of the major features in the plasmid is: T7 promoter - XhoI - SV40 promoter - HTLV-1 LTR promoter - HIV-1 LTR enhancer - EcoRI/MCS/BglII - SV40 polyadenylation - T3 promoter - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1946: Terrific broth (ATCC medium 1743) with 100 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Ventura AM, Villa LL. Plasmid pSH: a strong mammalian expression vector. Biochem. Biophys. Res. Commun. 192: 867-869, 1993. PubMed: 8387291 |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze dried plasmid in E. coli |