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| 产品名称： | D2a |
|---|---|
| 商品货号： | TS148445 |
| Designations： | D2a |
| GenBank Number： | L10717 |
| Species： | Homo sapiens, human |
| Applications： | A 2.8 kb PstI fragment from the unique and untranslated 5 domain has been used as a probe. |
| Vector： | Construct size (kb): 12.39999961853027 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ITK Genomic copy number: unique Gene name: T-cell-specific tyrosine kinase; homolog of mouse T-cell itk/tsk tyrosine kinase Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q31-q32 Tissue: thymus Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 9.4 Cross references: DNA Seq. Acc.: L10717 Insert lengths(kb): 9.399999618530273 Tissue: thymus Gene product: T-cell-specific tyrosine kinase; homolog of mouse T-cell itk/tsk tyrosine kinase ITK |
| Insert Size (kb)： | 9.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 3.6, 2.5; EcoRI--4.8, 3.1, 2.5, 2.0; PstI--9.2, 2.8, 0.56; XbaI--5.9, 3.8, 1.8, 0.7; HindIII--4.5, 4.3, 2.5, 1.1. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--5.92; BglI--2.13; BglII--4.67; EcoRI--1.95; EcoRV--4.48; HindIII--1.32, 2.43; KpnI--1.90; PstI--2.83, 3.39; SstI--2.19, 3.11; XbaI--5.26, 5.95. Contains the complete coding sequence (nt 2024-3886). A 2.8 kb PstI fragment from the unique and untranslated 5 domain has been used as a probe. |
| References： | Gibson S, et al. Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. Blood 82: 1561-1572, 1993. PubMed: 8364206 |