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| 产品名称： | pF5-1 |
|---|---|
| 商品货号： | TS148455 |
| Designations： | pF5-1 |
| GenBank Number： | M73047 |
| Species： | Homo sapiens, human |
| Depositors： | B Tomkinson |
| Vector： | Construct size (kb): 3.640000104904175 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.9399999976158142 Tissue: fibroblast Gene product: tripeptidylpeptidase 2 TPP2 |
| Insert Size (kb)： | 0.940 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.92; HindIII--2.7, 0.94; BamHI--3.5, 0.24; BglII--3.7; PvuII--2.4, 1.25. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.05; BglII--0.23; BamHI--0.80. Nucleotides 1-921 of the insert correspond to nt 2136-3056 of the sequence record. Encodes an internal region of the protein. One of 4 clones (pI7-11, pF5-1, pA2-10, pA2-13, ATCC 65988-65995) that together contain the complete coding sequence. Cloned in the antisense direction. The 3 EcoRI site was derived from the polylinker. |
| References： | Tomkinson B. Nucleotide sequence of cDNA covering the N-terminus of human tripeptidyl peptidase II. Biomed. Biochim. Acta 50: 727-729, 1991. PubMed: 1840501 Tomkinson B, Jonsson AK. Characterization of cDNA for human tripeptidyl peptidase II: the N-terminal part of the enzyme is similar to subtilisin. Biochemistry 30: 168-174, 1991. PubMed: 1670990 Birgitta Tomkinson, personal communication |