微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pSYX34lacZalpha |
|---|---|
| 商品货号： | TS148464 |
| Designations： | pSYX34lacZalpha |
| Depositors： | S Xu |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5000000000000000 Vector: pSYX34lacZalpha (plasmid) Construction: pSC101, pACYC184, pUC19 Marker(s):cmlR Construct size (kb): 6.5 Features: marker(s): cmlR replicon: pSC101 MCS: HindIII...SacI coding sequence: lacZalpha |
| Applications： | vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; PstI--5.4. A low copy-number vector compatible with plasmids having pMB1 or p15A origins of replication. Permits blue/white screening for recombinants. The copy number is estimated at 10 copies per cell. Recommendation for verification: BamHI; HindIII; PstI. |
| Media： | ATCC Medium 1065 (see below) plus 30.0 mcg/ml chloramphenicol ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
|
| Growth Conditions： | Temperature: 37.0°C |
| References： | Xu SY, Fomenkov A. Construction of pSC101 derivatives with Camr and Tetr for selection or LacZ for blue/white screening. BioTechniques 17: 57, 1994. PubMed: 7946313 Shuang-yong Xu, personal communication |
| Shipped： | freeze-dried |