| 产品名称: | pBR0.9EZZ probe C |
|---|---|
| 商品货号: | TS148495 |
| Designations: | pBR0.9EZZ probe C |
| Species: | Homo sapiens, human |
| Depositors: | M Lefranc |
| Vector: | Construct size (kb): 3.599999904632568 |
| Insert: | DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: immunoglobulin heavy chain gamma IGHG@* |
| Insert Size (kb): | 0.900 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.7, 0.9; EcoRI--2.7, 0.9; HindIII--3.3, 0.3; BamHI--3.6; EcoRI/HindIII--2.55, 0.61, 0.41, 0.3. The probe detects a 9.7 kb BamHI genomic fragment, a 6.5 kb and 8.9 kb HindIII genomic fragment, and a 9.2 SacI genomic fragment. This was isolated from lambdaEZZ from a region about 13 kb 3 to IGHG3. This region and similar ones downstream of IGHG genes appear to be highly homologous spots of recombination involved in multigene deletions of the IGH constant region locus. |
| References: | Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231 |