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| 产品名称： | pTH4 TRP1 --> HIS3 converter |
|---|---|
| 商品货号： | TS148522 |
| Designations： | pTH4 TRP1 --> HIS3 converter |
| Depositors： | FR Cross |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.7
Vector: pTH4 (phagemid)
Construction: pBC KS+
Marker(s): cmlR, kanR, HIS3
gene disruption cassette: tr:HIS3/kanR
replicon: f1 replicon: pMB1 restriction site: SmaI, EcoRI restriction site: XhoI |
| Applications： | YI-type (integrating) shuttle vector host modification marker swap vector TRP1 --> HIS3 |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; HindIII--4.7, 1.8, 0.2; EcoRI/XhoI--3.5, 3.3. To convert the host phenotype from TRP1 to HIS3, transform with the XhoI+EcoRI digested vector and select for His+ transformants. Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates. In most but not all cases the frequencies of successful convertants are greater than 30%. A marker swap vector designed to change the S. cerevisiae host phenotype by one-step gene disruption of the TRP1 gene with the HIS3 and kanR markers. When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. Vector was constructed by replacing an internal EcoRV fragment of TRP1 with a SmaI fragment containing the HIS3 and kanR coding sequences. HIS3 and TRP1 are in the same orientation. |
| Media： | ATCC® Medium 2994: Medium 1065 plus Kanamycin 50µg/ml plus Chloramphenicol 25µg/ml |
| Growth Conditions： | Temperature: 37°C |
| References： | Cross FR. Marker swap plasmids: convenient tools for budding yeast molecular genetics. Yeast 13: 647-653, 1997. PubMed: 9200814 |
| Related Products： | component of:ATCC 87561 |
| Shipped： | frozen |