| 产品名称: | NT18 NT-18 |
|---|---|
| 商品货号: | TS148566 |
| Designations: | NT18 NT-18 |
| GenBank Number: | M12056 |
| Species: | Mus musculus, mouse |
| Depositors: | RM Perlmutter |
| Vector: | Construct size (kb): 4.800000190734863 |
| Insert: | DNA: cDNA Insert lengths(kb): 2.09500002861023 Tissue: thymus Gene product: lymphocyte protein tyrosine kinase(tyrosine kinase lck) Lck |
| Insert Size (kb): | 2.095 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): StuI--2.9, 1.55; SmaI--3.1, 0.75, 0.6; BamHI--3.8, 0.65; PstI--3.4, 0.9, 0.2; EcoRI--2.75, 1.8. The insert contains the following restriction sites (approximate kb from the 5 end): StuI--0.14, 1.70; PstI--0.18, 1.05; BamHI--0.61; EcoRI--1.75. Detects genomic BamHI fragments of approximately 22, 9.2, and 1.6 kb in normal mouse cells, with an extra band of 5.0 kb in LSTRA cells. Contains the complete coding sequence (nt 164-1693). A StuI digest excises the intact coding sequence. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Marth JD, et al. A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murint T cell lymphoma LSTRA. Cell 43: 393-404, 1985. PubMed: 2416464 |