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| 产品名称： | YIp351 |
|---|---|
| 商品货号： | TS148610 |
| Designations： | YIp351 |
| Depositors： | A Tzagoloff |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4099998474121090 Vector: YIp351 (plasmid) Promoters: Promoter lac Construction: YEp351 Marker(s):LEU2,ampR Construct size (kb): 4.409999847412109 Features: insert detection: lacZ marker(s): ampR, LEU2 promoter: lac replicon: pMB1 terminator: none enhancer: none |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; XbaI--4.4; PstI--4.4; HindIII--4.4. This is an Escherichia coli/Saccharomyces integrative shuttle vector with multiple cloning sites. Constructed from YEp351 (ATCC 37672) by deleting all 2 micron circle sequences by digestion with HpaI and AatII (nt 2259-3492). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305 |
| Shipped： | freeze-dried |