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微信小章| 产品名称： | BT142 mut/- |
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| 商品货号： | TS148650 |
| Organism： | Homo sapiens, human |
| Tissue： | Brain |
| Cell Type： | Neural |
| Product Format： | frozen |
| Morphology： | Neurosphere |
| Culture Properties： | Suspension |
| Biosafety Level： | 1 It is the responsibility of the investigator to determine appropriate safety procedures for use with this material. As a reference, laboratory safety is discussed in the publication Biosafety in Microbiological and Biomedical Laboratories and can be accessed by searching "BMBL" at www.cdc.gov.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Oligoastrocytoma Grade III |
| Age： | 38 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | Liquid Nitrogen Vapor Phase |
| Images： |  |
| Clinical Data： | 38 years Male Caucasian Oligoastrocytoma Grade III |
| Comments： | Point mutations in isocitrate dehydrogenase I (IDH1) and IDH2 are found in majority of grade II and III gliomas. R132H is the most common IDH1 substitution found in gliomas. BT142 mut/- xa0contains a homozygous IDH1 R132H mutation, which originated from a heterozygous IDH1 R132H BT142 cells. The cells grow as phase-bright, smooth spheres. The neurospheres should not get too big, ragged or dark as this is a sign of unhealthy, dying cells. The cells should be passaged when the neurospheres are 200 to 400 µm in size. |
| Complete Growth Medium： | There are two options for the base medium for this cell line. Option 1: NeuroCult NS-A Proliferation kit (Catalog No. 5751, Stem Cell Technologies) Option 2: DMEM/F12 (1:1) (Catalog No. 30-2006, ATCC) with an additional 0.9% glucose, 4 mM L-glutamine (Catalog No. 30-2214, ATCC), 25 µg/mL insulin, 100 µg/mL transferrin, 20 nM progesterone, 15 µM putrescine and 30 nM selenite To make the complete growth medium, add the following supplements to either options of the base medium (see above):
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| Subculturing： | The cells grow as phase-bright, smooth spheres. The neurospheres should not get too big, ragged or dark as this is a sign of unhealthy, dying cells. The cells should be passaged when the neurospheres are about 200-400 μm in size. Volumes used in this protocol are for 75 cm2 flask.
Note: If accurate cell count is necessary, Accumax (Catalog No. AM105, Innovative Cell Technologies) can be used; however, the cells may take some time to recover from an enzymatic dissociation. xa0 xa0 |
| Cryopreservation： | Freeze medium: Complete growth medium (90%) supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | This cell line was deposited by Samuel Weiss, Ph.D. and Gregory Cairncross, Ph.D. of the University of Calgary |
| References： | Luchman HA et al. An in vivo patient-derived model of endogenous IDH1-mutant glioma. Neuro Oncol. 14:184-191, 2012. PubMed: 22166263 |