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微信小章| 产品名称： | pDV2SP0.5 |
|---|---|
| 商品货号： | TS148654 |
| Designations： | pDV2SP0.5 |
| GenBank Number： | X15207 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.200000047683716 |
| Insert： | DNA: genomic Insert lengths(kb): 0.5759999752044678 Tissue: Burkitts lymphoma LY67 cell line Gene product: T-cell receptor delta variable region V2 TCRDV2* Alleles: A1, A2, A3 |
| Insert Size (kb)： | 0.576 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.54; PstI/SacI--3.3; PstI--3.3; SacI--uncut; EcoRI--3.3; HindIII--3.3. This is a 576 bp SmaI/PstI fragment containing the complete coding sequence for the germline TCRDV2 gene. The promoter region of TCRDV2 has been sequenced. The A1 and A3 alleles of the BamHI polymorphism for the Chinese population have a combined frequency of 0.85. Hybridizes to a human genomic EcoRI fragment of 12.5 kb, a HindIII fragment of 4.0 kb, and an XbaI fragment of 3.6 kb. |
| References： | Dariavach P, Lefranc MP. First genomic sequence of the human T-cell receptor delta2 gene (TRDV2). Nucleic Acids Res. 17: 4880, 1989. PubMed: 2526321 Chuchana P, et al. BamHI restriction fragment alleles of the human T-cell receptor delta (TRD) variable region V2. Nucleic Acids Res. 17: 3622, 1989. PubMed: 2566979 Dariavach P, Lefranc MP. The promoter regions of the T-cell receptor V9 gamma (TRGV9) and V2 delta (TRDV2) genes display short area repeats but no TATA box. FEBS Lett. 256: 185-191, 1989. PubMed: 2806544 |