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| 产品名称： | pHPV-16 purified plasmid DNA |
|---|---|
| 商品货号： | TS148669 |
| Designations： | pHPV-16 purified plasmid DNA |
| GenBank Definition： | Human papillomavirus type 16 variant, complete sequence |
| GenBank Number： | U89348.1 |
| Species： | human papilloma virus type 16 |
| Depositors： | JE Marich, Syngene, Inc |
| Vector： | Size of Construct (kb): 10.866
Excise insert: BamHI+BglI (insert fragment will include 205 nt of the vector). Vector: pBluescript SK-
Size of intact vector (kb): 2.960
Markers: ampR
Vector ends: XhoI |
| Insert： | Insert size (kb): 7.904
Genome: human papilloma virus type 16
Gene name: genome, full-length
Contains complete coding sequence?: U
Type of DNA: genomic
Insert ends: BamHI |
| Insert Size (kb)： | 7.904 |
| Related Products： | ATCC 45113 ATCC VR-3240SD |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | 200 ng purified plasmid DNA, dried. Rehydrate with TE or water. |
| Comments： | Cloned from human papilloma virus type 16 by linearizing the virus at the unique BamHI site.
Full-length.
The insert contains the following restriction sites (nt from the vector KpnI site nt according to GenBank accession K02718): PstI--654 6791, 870 7007, 2646 879, 5463 3696, 6526 4759, 7009 5242; KpnI--2651 884, 7148 5381; EcoRI - 685 6818, 1320 7453. BamHI+BglI will excise the insert with 205 nucleotides of the vector. Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.7, 2.7; EcoRI--7.0, 3.6, 0.6. |
| Classification： | Papovavirus, Papillomavirus, recombinant |
| References： | James E Marich, personal communication |