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| 产品名称： | p414 GALS |
|---|---|
| 商品货号： | TS148695 |
| Designations： | p414 GALS |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.4289999008178710 DESCRIPTION OF VECTOR: Intact vector size: 5.429 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS414, GALS promoter Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): TRP1, ->, 200-1213 terminator: CYC1, ->, 1895-2155 MCS: XhoI...SpeI, ->, 2155-2212 promoter for expression: GALS, marker(s): ampR, centromere: CEN6/ARSH4, ->, 4915-5429 Vector: p414 GALS (plasmid) Promoters: Promoter for expression GALS Construction: pRS414, GALS promoter Marker(s):TRP1,ampR Construct size (kb): 5.428999900817871 Features: marker(s): TRP1 marker(s): ampR promoter for expression: GALS MCS: XhoI...SpeI terminator: CYC1 centromere: CEN6/ARSH4 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--5.4; SacI/XbaI--3.2, 1.8, 0.41. The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |