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| 产品名称： | pSP65:HSV #11 partial length clone of hop stunt viroid |
|---|---|
| 商品货号： | TS148844 |
| Designations： | pSP65:HSV #11 partial length clone of hop stunt viroid |
| Species： | Hop stunt viroid |
| Depositors： | RA Owens, Beltsville Agricultural Research Center |
| Applications： | To use as a template, the plasmid must be linearized with either PstI or HindIII. |
| Vector： | Name of construct: pSP65:HSV #11 Excise insert: BamHI+PstI Size of construct (kb): 3.26 Markers: ampR Name of vector: pSP65 Intact vector size: 3.010 Type of vector: plasmid Vector end: BamHI Vector end: PstI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: SP6 replicon: pMB1 terminator: none |
| Insert： | Genome: hop stunt viroid Gene name: genome, partial Contains complete coding sequence?: U Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.26 |
| Insert Size (kb)： | 0.260 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal SstII and XhoI sites. Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.0, 0.29; PstI--3.2; HindIII--3.2. To use as a template, the plasmid must be linearized with either PstI or HindIII. A partial length (about 260 bp) fragment of Hop Stunt Viroid cDNA was cloned into BamHI/PstI of pSP65; noninfectious. Constructed by R.W. Hammond. E. Shikata provided the original Hop Stunt Viroid cDNA. |
| Classification： | Hostuviroid |
| References： | Ohno T, et al. Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy. Nucleic Acids Res. 11: 6185-6197, 1983. PubMed: 6312412 Robert A Owens, personal communication |